- Original Article
- The optimal conditions to improve retrovirus-mediated
transduction efficiency to NIH 3T3 cells
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Jun Ah Lee, Kang-Min Lee, Hyun Jae Lee, Yun Jeong Lee, Dong Ho Kim, Jung Sub Lim, Kyung-Duk Park
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Clin Exp Pediatr. 2007;50(10):1011-1017. Published online October 15, 2007
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Purpose : We tried to assess the optimal conditions to improve low transduction efficiency and their effect on target cells.
Methods : Cultured NIH 3T3 cells were incubated with retroviral vectors bearing an enhanced green fluorescent protein (eGFP) gene. We varied the ratio of viral vectors to target cells (1:1-1:8) and the number of transfections (X1, X2), and compared transduction... |
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